Preliminary study on fluorimetric RT-PCR in estimating postmortem interval in rats
- VernacularTitle:荧光标记RT-PCR技术在实验动物死亡时间推断中的应用
- Author:
Fangcheng ZHU
- Publication Type:Journal Article
- Keywords:
forensic pathology;
reverse transcriptase-polymerase chain reaction;
fluorescence;
glyceraldehyde phosphate dehydrogenase;
postmortem interval
- From:Journal of Third Military Medical University
2003;0(18):-
- CountryChina
- Language:Chinese
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Abstract:
Objective To investigate the application of fluorimetric reverse transcriptase-polymerase chain reaction(RT-PCR) to estimate postmortem interval(PMI)in rats.Methods A total of 44 rats were killed by cervical dislocation and placed in a chamber with temperature-controlling system at 20℃ for 0 to 8 d postmortem.Changes of the relative content of glyceraldehyde phosphate dehydrogenase(GAPDH) mRNA in the brain and liver were examined by fluorimetric RT-PCR.Results The amplification of GAPDH mRNA could be detected within 7 d in the brain and 2 d in the liver postmortem respectively,in a fashion of decreasing.Conclusion Fluorimetric RT-PCR can detect the time-dependent degradation of GAPDH mRNA in the brain and the liver,so it maybe provide a new index for estimating PMI.
