Effect of perilla frutescens(L?) Britt?oil on cytokines and renal calcium oxalate crystallization formation in rats
- VernacularTitle:苏子油对大鼠体内细胞因子及肾草酸钙结晶形成影响的研究
- Author:
Jun WANG
- Publication Type:Journal Article
- Keywords:
urinary calculi;
calcium oxalate;
?-Linolenic acid;
cytokines
- From:Journal of Third Military Medical University
2003;0(18):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the possible role of cytokines interleukin(IL)-1?,IL-1?,IL-6 and TNF? in urinary stone formation and the inhibitory effect of ?-Linolenic acid on these cytokines and renal calcium oxalate crystallization formation in rats.Methods Forty-five male adult Wistar rats were divided receive normal forage and water as negative control(Ⅰ),normal forage and water containing 1% ethylene glycol and 1% muriat to induce renal stone as group Ⅱ,or normal forage and water containing 1% ethylene glycol and 1% muriat and l0 g?kg~(1)?d~(-1) perilla frutescens(L?) Britt?oil as group Ⅲ for 8 weeks.Then all rats were sacrificed and the renal tissue calcium,the urinary calcium,the calcium oxalate crystallization and urinary oxalate and serum IL-1?,IL-1?,IL-6 and TNF-? were detected.RMCs were pretreated with 10 ?g/L IL-6,10 ?mol/L EPA+10 ?g/L IL-6,50 ?mol/L EPA+10 ?g/L IL-6,or absolute alcohol for 24 h.RMCs without pretreatment served as normal control.The PGE_2 concentration in RMC supernatant was measured by radioimmunoassay.Results As compared with group Ⅱ,the hydronephrosis and parenchymal swelling were obviously slight,and calcium oxalate crystalization,renal tissue calcium,urinary calcium excretion,blood urea nitrogen and creatinine were significantly lower in group Ⅲ(P
