The Simultaneous Determination of Gatifloxacin and Tinidazole by HPLC.
- VernacularTitle:高效液相色谱法同时测定加替沙星和替硝唑的含量
- Author:
Rong BAI
;
Xia LIU
- Publication Type:Journal Article
- Keywords:
Gatifloxacin;
Tinidazole;
HPLC
- From:
Journal of Medical Research
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a reversed HPLC method to determine the contents of gatifloxacin and tinidazole simultaneously. Methods ODS C_ 18 column (4.6mm?250mm,5?m) was used as an analytical column. The mobile phase consisted of 15% acetonitrile, 25% of methanol and 60% of 0.05mol/L KH_2PO_4 buffer,which contained 0.5% triethylamine and was adjusted to pH 3.5 by phosphoric acid. The flow rate was 1ml/min. The detection wavelength was 318nm. The column temperature was 30℃. 20?l of diluted sample solution was injected to the sampler. Results The calibration curve of gatifloxacin was linear in the range of 2~20?g /ml (r=0.999 6) ,and that of tinidazole was 2~20 ?g /ml (r=0.999 9). The recovery rates of them were 100.1% and 100.4%,respectively. RSDs of both drugs were less than 1.1 %. Conclusions The method is simple,accurate and sensitive and can be used to determine the two components simultaneously.
