Antiturbulent effect of the third-generation anti-HCV ELISA kits
- VernacularTitle:第三代抗-HCVELISA试剂盒抗干扰能力分析
- Author:
Li JIANG
;
Siyang PAN
;
Yanqiu LIU
- Publication Type:Journal Article
- Keywords:
Hepatitis antibodies;
Enzyme-linked immunosorbent assay;
False positive reactions
- From:
Chinese Journal of Primary Medicine and Pharmacy
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine antiturbulent effect of the third-generation anti-HCV ELISA kit.Methods Anti-HCV ELISA kits from domestic and foreign manufacturer were used to detect the samles.Thost samples with positive results obtained using different anti-HCV ELISA kits from five manufacturers were further tested by HCV BLOT to determine which result would be true.Then RT-PCR would be done on the samples with anti-HCV “Indeterminate” by HCV BLOT.Results False positive rate of five kinds of anti-HCV ELISA kits on 268 selected weak positive samples were 0.37%～12.68%.The results from different anti-HCV ELISA kits showed markedly inconsistent.If any two kinds were combined to detect the samples,the false positive rates declined 0～2.2%(P
