Study of realgar induced apoptosis of eosinophils from bronchoalveolar lavage fluid in asthmatic guinea pigs
- VernacularTitle:雄黄诱导哮喘豚鼠肺泡灌洗液中嗜酸细胞凋亡的研究
- Author:
Jide XU
;
Zhixiang HUANG
;
Shaowei YANG
;
Zhonghui WEN
;
Ruifang ZENG
- Publication Type:Journal Article
- Keywords:
realgar;
bronchoalveolar lavage fluid;
eosinophils;
apoptosis;
asthma
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2004;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the realgar induced the apoptosis of eosinophils from bronchoalveolar lavage fluid in asthmatic guinea pigs and investigate the mechanism that realgar treated asthma.METHODS: The morphology of apoptosis of eosinophils was observed by Giemsa staining and electron microscope.The rate of apoptosis of eosinophils was assayed by the flow cytometry.RESULTS: The characteristic changes of the apoptosis in both light microscope and electron microscope were shown after 6 hours treatment of realgar.Flow cytometry showed that the rate of apoptosis of the eosinophils was increased with both increasing realgar concentration and prolonging realgar action time to the cells.CONCLUSION: Realgar promotes the apoptosis of eosinophils from bronchoalveolar lavage fluid in asthmatic guinea pig.Realgar induced the apoptosis of eosinophils is one of the causeses for asthmatic treatment.
