Effect of CCK-8 on IL-1?-induced RSC-364 proliferation
- VernacularTitle:CCK-8对IL-1?诱导大鼠滑膜细胞株RSC-364增殖的影响
- Author:
Dongyan HAN
;
Bin CONG
;
Jinrong XU
;
Shujin LI
;
Chunling MA
;
Zhiyu NI
- Publication Type:Journal Article
- Keywords:
rheumatoid arthritis;
synovial cell;
IL-1?;
CCK-8
- From:
Chinese Pharmacological Bulletin
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects and mechanisms of CCK-8 on IL-1? induced proliferation of RSC-364, a rat fibroblast-like synovial cell line. Methods MTT colorimetric assay and Western blot were used to measure cell proliferation and p38MAPK phosphorylation level to elucidate the mechanism of CCK-8 in IL-1? induced RSC-364 proliferation. Results CCK-8 significantly inhibited IL-1?-induced RSC-364 proliferation at 10 -12 , 10 -10 , 10 -8 , 10 -6 mol ? L -1 , and IL-1?-activated p38MAPK activity at 10 -10 , 10 -8 , 10 -6 mol?L -1 in a dose-dependent manner. The effect of CCK-8 was blocked by CR1409 (a CCKA-receptor antagonist) and CR2945 (a CCKB-receptor antagonist). Conclusion CCK-8 inhibits IL-1?-induced RSC-364 proliferation, probably by reducing p38MAPK activity through CCKA and CCKB receptors.
