Purification of a small peptide to inhibit platelet aggregation from Agkistrodon acutus venom
- VernacularTitle:尖吻蝮蛇毒小分子多肽的分离及抗血小板聚集作用
- Author:
Danqing LEI
;
Xianli ZHOU
;
Yingxin LI
- Publication Type:Journal Article
- Keywords:
agkistrodon acutus venom;
purification;
platelet aggregation inhibitor
- From:
Chinese Pharmacological Bulletin
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To purify a small platide inhibiting platelet aggregation from Agkistrodon Acutus snake venom,and to identify its physical and chemical properties and its effects on the platelet aggregation stimulated by ADP, collagen, and thrombin. Methods Extract snake venom through Superdex 75 gel filtration, ultrafiltration and DEAE-Sepharose CL-6B ionexchange. The purified product was identified by HPLC C_ 18 . The molecular weight was determined by SDS-polyacrylamid gel electrphoresis. Platelet aggragation was measured by nephelometry. Results The molecular weight of the peptide was 7 862 u and its purified from Agkistrodon Acutus snake venom is oelectric point was pH 4.29. This peptide dose-dependently inhibited the platelet aggregation induced by ADP,collagen,and thrombin. Conclusion The method has been proved to be successful for the purification of low molecular weight peptide fraction that inhibits platelet aggregation.
