Protective effect of puerarin on anoxia-reoxygenation induced cerebral cell injury in culture
- VernacularTitle:葛根素对培养脑细胞缺氧/再给氧损伤的保护作用
- Author:
Yan WU
;
Huayin WAN
- Publication Type:Journal Article
- Keywords:
puerarin;
cerebral cells;
anoxia-reoxygenation;
ultrastructure;
calcium;
membrane fluidity
- From:
Chinese Pharmacological Bulletin
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the protective effect of puerarin(PUE) on cultured cerebral cells injured by anoxia-reoxygenation. Methods The anoxia-reoxygenation injury model were developed, anoxia for 60 min and reoxygenation for 30 min. the effect of PUE on cerebral ultrastructure was observed.[Ca 2+ ]_i was estimated with Adherent Cell Analysis and Sorting 570(ACAS 570) Laser Cytometer and measured with fluorescent dye Fura-2-AM, the lipid fluidity of cellular membrane was determined by fluorescence polarization technique. Results PUE improved the ultra-structure of cerebral cells, dose-dependently decreased[Ca 2+ ]_i and increased the lipid fluidity of cellular membrane. PUE markedly reduced the chromaticity value of pseudo-colour graphic model of Ca 2+ . Conclusion Puerarin has protective effect on anoxia-reoxygenation induced cerebral cell injury. The effect may be mediated by decreasing[Ca 2+ ]_i and increasing the lipid fluidity of cellular membrane.
