Expression of recombined human immunoglobulin ? light chain 022 and TOM1 mRNA in omental adipose tissues of patients with type 2 diabetes and insulin resistance
- VernacularTitle:人类重组免疫球蛋白?轻链022与TOM1基因在2型糖尿病胰岛素抵抗患者网膜脂肪组织的表达
- Author:
Weimin ZHAO
- Publication Type:Journal Article
- Keywords:
diabetes mellitus,type 2;
insulin resistance;
adipose tissue;
immunoglobulins lambda-chain;
TOM1
- From:
Academic Journal of Second Military Medical University
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of recombined human immunoglobulin ? light chain 022 and TOM1 gene and to determine the protein expression of HSIGVL022 in omental adipose tissues of patients with type 2 diabetes and insulin resistance(T2DM-IR).Methods: Fat tissues from greater omentum of T2DM-IR patients and normal controls were obtained.The mRNA levels of recombined human immunoglobulin ? light chain 022 and TOM1 were measured by fluorescent quantitative RT-PCR;expression of HSIGVL022 protein was identified by immunohistochemistry.Results: The mRNA levels of HSIGVL022 and TOM1 in T2DM-IR patients were(34 140?6 160) copy/million house-keeping genes and(4 440?617) copy/million house-keeping genes,respectively;those in control group were(5 930?661) copy/million house-keeping genes and(1 360?82) copy/million house-keeping genes,respectively.There were significant difference between the 2 groups(P
