Influence of agouti signal protein on melanocyte function in skin autograft
- VernacularTitle:刺鼠信号蛋白对自体移植皮片中黑素细胞功能的影响
- Author:
Chunyu XUE
- Publication Type:Journal Article
- Keywords:
agouti signal protein;
?-melanocyte-stimulating hormone;
tyrosinase;
skin transplantation;
hyperpigmentation
- From:
Academic Journal of Second Military Medical University
2001;0(09):-
- CountryChina
- Language:Chinese
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Abstract:
Objective:To study the influence of agouti signal protein(ASIP) on melanocyte function in skin autograft,so as to understand the cause of hyperpigmentation in skin autograft.Methods: Guinea pigs were used to establish a skin autograft hyperpigmentation model.The skin autografts in model animals were injected with ASIP or normal saline(control).RT-PCR technique was used to detect the tyrosinase mRNA expression in melanocytes of skin autografts and Masson-Fontana staining technique was used to detect the melanin contents in skin autografts in ASIP treatment group;and the results were compared with those of control group(n=13) and normal guinea pigs(n=5).Results: The expression of tyrosinase mRNA and the melanin content in skin autografts in ASIP treatment group were both lower than those of control group and normal guinea pigs((P
