Inhibitory effect of Bcl-2 short hairpin RNA on growth of BEL-7402 and Caco2 cells
- VernacularTitle:Bcl-2短发夹状RNA对BEL-7402、Caco2细胞的生长抑制作用
- Author:
Dongmei HE
;
Huan ZHANG
;
Gexiu LIU
- Publication Type:Journal Article
- Keywords:
Bcl-2;
shRNA;
RNAi;
BEL-7402 cells;
Caco2 cells
- From:
Academic Journal of Second Military Medical University
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of Bcl-2 shRNA on the growth of liver carcinoma cell line BEL-7402 and colorectal carcinoma cell line Caco2.Methods: Bcl-2 shRNA was cloned into Pgenesil-1 plasmid labeled with fluorescent protein and the product was transfected into BEL-7402 and Caco2 cells with Lipofectamine 2000.The study also included shRNA as negative control,Pgenesil-1,Lipofectamine and blank control groups.Transfected cells were visualized by inverted fluorescent microscope and then assayed by flow cytometry.The expression levels of Bcl-2 protein were assayed by Western-blot and cell proliferation was measured by MTT method.Results: There was no difference in transfection rate among cells in Bcl-2 shRNA,shRNA and Pgenesil-1 vector groups.Western-blot assay showed that the expression levels of Bcl-2 protein in BEL-7402 and Caco2 cells were significantly decreased in Bcl-2 shRNA group compared with those in other 4 groups(P
