Isolation and purification of water-soluble polysaccharide MP-Ⅰ from Mytilus coruscus and study on its in vitro anti-tumor activity
- VernacularTitle:贻贝水溶性多糖MP-Ⅰ的分离纯化及体外抗肿瘤活性研究
- Author:
Hongli XU
- Publication Type:Journal Article
- Keywords:
mussels;
polysaccharides;
isolation and purification;
antineoplastic drugs
- From:
Academic Journal of Second Military Medical University
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To separate and purify polysaccharide MP-Ⅰ from Mytilus coruscus and to investigate its in vitro anti-tumor activity.Methods: The crude polysaccharide was extracted from Mytilus coruscus with hot water and the protein was removed using Sevage method.The polysaccharide MP-Ⅰ was purified using DEAE-Sepharose and Sepharose CL-6B column chromatography.Monosaccharides analysis was carried out using thin-layer chromatography and gas chromatography.The in vitro anti-tumor activity of MP-Ⅰwas assessed using MTT method.Results: The yield of MP-Ⅰwas 2.14%.MP-Ⅰ is mainly constituted by glucose.MP-Ⅰ(at 0.5,0.1,0.02 mg/ml) had different degrees of inhibitory effects on HO-8910,MCF-7, K562,and SMMC-7721 tumor cells in vitro(P
