Protective effect of activated AMP-activated protein kinase on palmatic acid-induced damage of human umbilical vein endothelial cells
- VernacularTitle:激活的一磷酸腺苷活化蛋白激酶对软脂酸诱导的血管内皮细胞损伤的保护作用
- Author:
Ying CHENG
- Publication Type:Journal Article
- Keywords:
AMP-activated protein kinase;
5-amino-4-imidazolecarboxamide-riboside;
metformin;
pioglitazone
- From:
Academic Journal of Second Military Medical University
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the protective effect of activated AMP-activated protein kinase(AMPK) on the human umbilical vein endothelial cell(HUVEC) damage induced by palmatic acid(PA) and the related mechanism.Methods: HUVEC were divided into 8 groups according to the culture media(cultured for 24 to 72 h): blank control(conventional culture),300 ?mol/L PA,1 mmol/L aminoimidazole-4-carboxamide-1-beta-ribofuranoside (AICAR),PA+AICAR,2 mmol/L metformin,PA+metformin,10 ?mol/L pioglitazone,and PA+pioglitazone groups.Survival rates of HUVEC were determined by MTT at 24,48 and 72 h after culture.Western blot were performed to detect the expression of phospha-AMPK at 24 h after culture.Results: Compared with blank control group,cell survival rates in PA group were decreased by 58.95%,36.68% and 15.09% at 24,48 and 72 h(P
