Effects of peripheral blood derived dendritic cells on T cell activity in colorectal cancer patients.
- VernacularTitle:大肠癌患者外周血来源树突状细胞对T细胞活化作用的研究
- Author:
Jianxin WANG
;
Wenming CHEN
;
Jinwei LIU
- Publication Type:Journal Article
- Keywords:
Colorectal cancer;
Dendritic cells;
T lymphocyte proliferation
- From:
Chinese Journal of Practical Internal Medicine
2006;0(18):-
- CountryChina
- Language:Chinese
-
Abstract:
0.05).The stimulative index(SI)tested by MTT in MLR at stimulator-to-reactor cell ratio 1∶1,1∶10,1∶20,1∶40 and 1∶80 was 2.7,4.1,3.1,2.5 and 1.6,respectively.The proliferation of T cells stimulated by DCs could be detected at all stimulator-to-reactor ratio.Conclusion DCs could be successfully induced from peripheral blood monocyte in the patients with colorectal cancer by AIM-V serum-free media or by traditional FBS-contained media.AIM-V is more suitable for clinical immunotherapy because it can avoid the risk of allergic reaction for clinical purpose.AIM-V might take the place of serum-contained media to culture DCs.According to the results of MLR,DCs could stimulate allogenetic T cells proliferation at all stimulator-to-effector ratio,furthmore the optimal ratio is 1∶10 .
