Changes of serum IL-6 levels and pulmonary IL-6 mRNA expression after high-volume hemofiltration in pigs with multiple organ dysfunction syndrome
- VernacularTitle:早期高容量血液滤过后多器官功能障碍综合征猪血清IL-6浓度及肺IL-6 mRNA表达的变化及意义
- Author:
Bing WANG
- Publication Type:Journal Article
- Keywords:
multiple organ dysfanctious syndrome;
hemofiltration;
interleukin-6;
apoptosis
- From:
Academic Journal of Second Military Medical University
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of high-volume hemofiltration(HVHF) on serum levels of IL-6 and pulmonary expression of IL-6 mRNA in pigs with multiple organ dysfunction syndrome(MODS).Methods: Twenty-five pigs were randomly divided into model group,control group and HVHF group.Animals in control group were injected with normal saline,and those in the other 2 groups were treated with "a second hit" to establish MODS model,then animals in HVHF group was treated with HVHF for 3 days.Serum IL-6 levels were determined by ELISA and Real-time quantitative PCR was used to determine the expression level of IL-6mRNA.Lymphocyte apoptosis was detected by Annexin-Ⅴ/PI flow cytometry.Results: After HVHF,the serum indices associated with organ-function and the morbidity and mortality were much lower than those in MODS model group(P
