Inhibitory effect of zileuton on growth of pancreatic cancer cell lines.
- VernacularTitle:5-脂氧合酶抑制剂齐留通对胰腺癌细胞SW1990的生长抑制作用
- Author:
Guoxiong ZHOU
;
Shenbao WU
;
Jiefei HUANG
- Publication Type:Journal Article
- Keywords:
Pancreatic neoplasm;
Zileuton;
5-lipoxygenase
- From:
Chinese Journal of Practical Internal Medicine
2006;0(18):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the 5-lipoxygenase(5-LOX)expression in human pancreatic cancer cells and the inhibitory effect on growth of SW1990 by selective 5-LOX inhibitor zileuton in vitro.Methods The 5-LOX expression in pancreatic cancer cell was defected by immunocytochemistry and RT-PCR form May 10,2003 to May 25,2004.MTT and flow cytometry with AnnexinⅤ-FITC and propidium iodide(PI)staining and acridine orange(AO)staining were used to observe the effect of zileuton on the growth of SW1990cell line.Results The 5-LOX mRNA and protein expression was detectable in pancreatic cancer cell line by mmunohistochemistry and RT-PCR technique respectively.Zileuton could inhibit the growth of SW1990 cell line in a time-and dose-dependent manner.Conclusion Zileuton might inhibit the growth of SW1990 cell line in a time-and dose-dependent manner.
