Effect of decorin on proliferation and cell cycle of rabbit tendon cells in vitro
- VernacularTitle:核心蛋白聚糖对兔肌腱细胞增殖及细胞周期的影响
- Author:
Yan XIONG
;
Zhengzhi ZHANG
;
Xiaolan FU
- Publication Type:Journal Article
- Keywords:
decorin;
tendon cells;
cell cycle
- From:Journal of Third Military Medical University
2003;0(17):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate effect of decorin on proliferation and cell cycle of rabbit tendon cells in vitro so as to explore the role of decorin in tendon wound healing.Methods Tendon cells derived from the tissue of rabbits flexor tendon were harvested and cultured in vitro with decorin of 0.25,1.25,2.5,5 ?g/ml.After culture of 12,24 or 48 h,the cell proliferation rate was measured by MTT colorimetric determination.After 24-hour culture with 0.25?g/ml decorin,the morphology of tendon cells was obtained and the cell cycle distribution was detected by flow cytometer.Results The proliferation of tendon cells was inhibited after 12-hour culture and significantly increased after 24-hour culture with 0.25,1.25,2.5 ?g/ml decorin.However,5 ?g/ml decorin could increase the proliferation after 12-hour culture,increase after 24-hour culture,with no significant difference between 24 h and 48 h.decorin at 0.25 ?g/ml could significantly increase the cells at S phase and PI after 24-hour culture(P
