Expression of bone morphogenetic protein 7 and its potential role in diabetic rats
- VernacularTitle:骨形成蛋白7在糖尿病大鼠肾组织中的表达及意义
- Author:
Hong LIU
;
Lijing CHENG
;
Ming CHANG
;
Zhengnan GAO
- Publication Type:Journal Article
- Keywords:
Bone morphogenetic proteins;
Diabetic nephropathy;
Transforming growth factor beta
- From:
Chinese Journal of Nephrology
2005;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression of bone morphogenetic protein 7 (BMP-7) in different stages of diabetic rats and investigate the potential role of BMP-7 in diabetic nephropathy (DN). Methods Wistar rats were divided into diabetes mellitus(DM) group induced by intravenous injection of streptozotocin (65 mg/kg) , and normal control group injected with citrate buffer. The rats were sacrificed at day 30,60,90,120,150 and 180 following injection, respectively. The blood glucose,Scr, urinary albumin and urinary creatinine were measured in each rat before sacrifice. The renal pathological changes were examined with hematoxylin and eosin (HE),periodic acid-silver-methenamine staining (PASM) and protein expression of collagenⅣ(ColⅣ). The mRNA and protein expression of BMP-7 and TGF-?1 in kidney were detected by reverse transcription-polymerase chain reaction (RT-RCR) and immunohistochemical staining respectively, and were quantified by computer image analysis system. Results The levels of blood glucose and urinary albumin in diabetic group were remarkably higher than those in control group (P
