Expression and implication of tissue transglutaminase in kidney of diabetic nephropathy rats
- VernacularTitle:组织型转谷氨酰胺酶在糖尿病大鼠肾组织中的表达及意义
- Author:
Yahong LIU
;
Lijing CHENG
;
Zhengnan GAO
- Publication Type:Journal Article
- Keywords:
Transglutaminase 2;
Diabetic nephropathy;
Fibrosis
- From:
Chinese Journal of Nephrology
2005;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression of tissue transglutaminase (tTG) in renal tissues of diabetic nephropathy rats, and to investigate its contribution to the progression of diabetic nephropathy. Methods Streptozotocin-induced diabetic rats were sacrificed at 30 d, 60 d, 90 d, and 120 d respectively. Albuminuria excretion rate (AER), kidney weight index and serum creatinine (Scr) of the rats were measured. Hematoxylin and eosin (HE) staining and periodic acid-silver-methenamine (PASM) staining were used to observe the renal pathological changes. CollagenⅣ(ColⅣ) and soluble tTG protein in kidney were examined by immunohistochemistry,insoluble tTG by immunofluorescence. Expression of total tTG mRNA in kidney was detected by reverse transcription-polymerase chain reaction (RT-PCR). Results AER, kidney weight index and Scr of diabetic rats were all progressive during the period of the experiment. Compared with control group, the levels of ColⅣ, soluble tTG and insoluble tTG protein in kidneys of hyperglycemic rats were significantly increased at 30 d, 60 d, 90 d and 120 d(P
