Effects of morphine on Gi_2 protein in primary cultured hippocampal neurons
- VernacularTitle:吗啡对原代培养海马神经元内Gi_2蛋白水平的影响
- Author:
Qinghua WU
;
Qiang FU
;
Xinhua WANG
- Publication Type:Journal Article
- Keywords:
morphine;
hippocampus;
neurons;
fluoroimmunoassay;
guanine nucleotide binding protein, inhibited
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The molecular basis for opiate tolerance and dependence remains poorly understood despite extensive investigation in several preparations, including the hippocampus. Recent studies have implicated that the hippocampus played a central role in opiate tolerance, dependence and withdrawal. The current study is to explore the change in guanine nucleotide binding protein-inhabitant (Gi_2) protein in primary cultured hippocampal neurons with morphine treament. Methods The hippocampus was harvested from newborn Sprague-Dawley rats. Primary hippocampal neuronal cultures of 7 days in vitro were used and divided randomly into six groups (n=6), i.e. morphine treatment 4h group (M4), 8h group (M8), 16h group (M16), 24h group (M24), 48h group (M48) and control group (C). All morphine treatment groups were treated with morphine (10?mol/L). C group was treated with saline. The G protein levels were determined with immunofluorscence and laser scanning confocal microscope (LSCM) imaging techniques. Results Gi_2 protein levels in M16, M24 and M48 groups decreased significantly compared with that in C group (P0.05). Among M16, M24 and M48 groups, Gi_2 protein level was lowest in the M48 group. Conclusion The results indicated that Gi_2 protein levels decreased significantly in primary cultured hippocampal neurons with morphine treatment, which might be a potential molecular mechanism of opioid tolerance and dependence.
