Mutation analysis of tumor suppressor PTEN in sporadic breast carcinoma with PCR-SSCP
- VernacularTitle:乳腺癌组织PTEN基因突变的PCR-SSCP分析
- Author:
Hongyan ZHANG
;
Gang LI
;
Santai SONG
- Publication Type:Journal Article
- Keywords:
genes, PTEN;
breast neoplasms;
point mutation
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the mutation frequency and mutation type of tumor suppressor PTEN in sporadic breast carcinoma tissues, and to investigate the association of PTEN gene mutation and sporadic breast cancer. Methods Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) was used to amplify 9 exons of PTEN and to analyze the conformation polymorphism, then DNA sequencing methods was used to detect point mutation of PTEN gene nine exons of abnormal single strand conformation in breast carcinoma. Results One of 45 cases showed 24th condon A→C missense mutation in the exon2 of PTEN gene, and this mutation made Met to change to Ala in PTEN protein structure. Conclusion These data indicated the existence of PTEN mutation in sporadic breast cancer, but PTEN gene mutation rate is very low.
