Effect of IGF-1 on the InsR?、? gene expression and protein content in pyaemia rats
- VernacularTitle:脓毒症大鼠肝和骨骼肌胰岛素受体蛋白基因表达及其IGF-1的作用
- Author:
Huanlong QIN
;
Zhiguang GAO
;
Minfeng LIU
- Publication Type:Journal Article
- Keywords:
Pyaemia;
Insulin resistance;
IGF-1;
InsR?、?
- From:
Parenteral & Enteral Nutrition
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the influences of IGF-1 on the stress homone,InsR?、? gene expression and protein content in pyaemia rats.Methods:SD rats with abdominal infection models established by cecal ligation and perforation method were randomly divided into three groups: pyaemic group(n=10),parenteral nutrition group(PN group,n=10) and IGF-1 Group (n=10),and 10 SD rats were used as normal group(n=10).On the sixth day,Vena Cava blood was sampled to determine the level of glucose,insulin,glucagon,and IGF-1.In addition,the expression of the InsR?、? in liver and muscle were detected by immunohistochemistry and Real-Time PCR methods.Results:The plasma glucose,insulin,glucagon in the IGF-1 group were significantly lower than that of the pyaemic group and PN group(P
