Verapamil modulates LPS-induced cytokines production via inhibiting NF-?B activation in the liver of rats
- VernacularTitle:不同剂量维拉帕米对内毒素血症大鼠肝细胞因子表达和NF-?B活化的影响
- Author:
Gang LI
;
Xiaoping QI
;
Zhe XU
;
Che CHEN
;
Xiaodong YANG
;
Xiaoyu WU
;
Zheng SUN
;
Pei LI
;
Jieshou LI
- Publication Type:Journal Article
- Keywords:
Verapamil;
Endotoxin;
Acute hepatic lesion;
NF-?B
- From:Parenteral & Enteral Nutrition
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of verapamil on the endotoxin-induced cytokine production and nuclear factor kappa B(NF-?B) activation in the liver. Methods:Fifty six adult male Sprague-Dawley rats were randomly divided into seven groups: group A: saline;group B:endotoxin(10 mg/kg,intraperitoneally,i.p.);group C,D,E,F:endotoxin(10 mg/kg) after treatment with verapamil(1,2.5,5,10 mg/kg i.p.respectively),and group G:verapamil alone(10 mg/kg).Tumor necrosis factor(TNF-?),interleukin-6(IL-6), interleukin-10(IL-10) and NF-?B in the liver tissues and the serum levels of ALT and AST were investigated at 1 h afer LPS injection. Results:Endotoxin stimulated production of TNF-?,IL-6 and IL-10,and activated NF?B in the liver.Verapamil attenuated acute liver injury,down-regulated endotoxin-induced pro-inflammatory cytokine production,up-regulated ant-inflammatory cytokines production and inhibited NF-?B activation in the liver in a dose-dependent manner.Conclusion:Verapamil can attenuate acute liver injury by down-regulating the production of TNF-?,IL-6 and up-regulating IL-10 in the liver in a dose-dependent manner,possibly via inhibition of NF-?B.
