Study on the effect of growth hormone and chemotherapy in tumor bcaring micc
- VernacularTitle:生长激素对荷瘤小鼠化疗效果影响的实验研究
- Author:
Lin WANG
;
Suyi LI
;
Fangfang SHI
- Publication Type:Journal Article
- Keywords:
Recombinant human growth hormone;
Liver metastases tumor;
Animal model;
Chemotherapy sensitivity
- From:
Parenteral & Enteral Nutrition
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of recombinant human growth hormone(rhGH) and its combination with chemotherapy(5-FU) in tumor bearing mice.Methods:Hepatic metastases model were established in 615 mice by splenic injection of proventriculus squamous carcinoma cell(MFC).Among them,sixty mice were divided randomly into 6 groups(ten per group): control(NS),GH1,GH2,flurouracil(5-FU),flurouracil plus rhGH(GH1+5-FU) and flurouracil plus rhGH(GH2+5-FU).Body weights were measured every week.On the 28~(th) day,animals in each group were executed.The weight of body and liver was measured,and cell cycle of tumor DNA was determined by flow cytometry(FCM). Other 48 animals were divided randomly into 6 groups(eight per group) and treated with the same methods as above.They were fed till death to observe survival time. Results:Body weights in the GH1+(5-FU) and the GH2+5-FU group were increased than that of control group(P
