Effect of Bifidobacterium spent culture supernatant on Pseudomonas aeruginosa adhering to intestinal epithelial cells
- VernacularTitle:双歧杆菌培养上清对铜绿假单胞菌黏附肠上皮细胞影响的研究
- Author:
Jun CHEN
;
Peiyuan XIA
;
Guangxia XIAO
- Publication Type:Journal Article
- Keywords:
bifidobacterium;
adhesion;
pseudomonas aeruginosa;
IEC-6
- From:Journal of Third Military Medical University
2003;0(16):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of Bifidobacterium spent culture supernatant (SCS) on adhesion of Pseudomonas aeruginosa to intestinal epithelial cells. Methods The intestinal epithelial cells were cultured,then divided into three groups: control,Pseudomonas aeruginosa adhesion group,SCS and Pseudomonas aeruginosa adhesion group. The effects of SCS on cell viability,the number of adhering and invasive bacteria were evaluated with MTT assay and lysis-counting assay in 1,3,6 h. Results At 3 h after SCS treatment,the amount of Pseudomonas aeruginosa decreased significantly,and the number of adhering and invasive bacteria decreased by 71% and 87% respectively. Conclusion SCS could protect the intestinal epithelial cells by inhibiting the adhesion and invasion of Pseudomonas aeruginosa.
