A role of p38 mitogen-activated protein kinase in regulating expression of Peroxisome proliferator-activated receptors-? in rat mesangial cells
- VernacularTitle:p38MAPK对大鼠肾小球系膜细胞表达PPAR-?的影响
- Author:
Qianping WEI
;
Huacong DENG
;
Ji ZHAO
- Publication Type:Journal Article
- Keywords:
p38 mitogen-activated protein kinase;
peroxisome proliferator-activated receptors-?;
diabetic nephropathy;
mesangial cells
- From:Journal of Third Military Medical University
2003;0(16):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between p38MAPK and PPAR-?,and to study the role of p38MAPK and PPAR-? for diabetic nephropathy. Methods The expression of p38MAPK protein and PPAR-? mRNA was investigated in rat mesangial cells line HBZY-1 that was incubated respectively with 25 mmol/L glucose,100 nmol/L insulin,100 ?mol/L H_2O_2 and 100 mg/L AGEs. The relationship between p38MAPK and PPAR-? expression was studied by using SB203580,a specific inhibitor of p38MAPK. Results p38MAPK had significantly higher expression,while the expression of PPAR-? was significantly decreased in rat mesangial cells line HBZY-1 incubated with 25 mmol/L glucose,100 nmol/L insulin,100 ?mol/L H_ 2 O_ 2 and 100 mg/L AGEs respectively. PPAR-? activity was significantly reduced when p38MAPK was inhibited by SB203580. Conclusion p38MAPK is involved in development of diabetic nephropathy and may antagonize the expression of PPAR-?. PPAR-? activity might protect the function of kidney.
