Changes of ANF mRNA expression in the myocardial tissue of rat embryo and suckling induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin
- VernacularTitle:2,3,7,8-四氯二苯并二噁英作用下鼠胚及乳鼠心脏组织ANF mRNA的表达变化
- Author:
Lili SUN
;
Qing CHANG
;
Zhiqing LIANG
;
Changxu SHI
- Publication Type:Journal Article
- Keywords:
TCDD;
cardiac tissue;
ANF mRNA
- From:Journal of Third Military Medical University
2003;0(16):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes of ANF mRNA expression in the myocardial tissues of rat embryo and neonate induced by 2,3,7,8-TCDD so as to determine if TCDD can cause injuries of cardiac functions. Methods SD rat embryos were divided into 4 groups respectively exposed to TCDD at the dose of 0.05,0.5,5,10 ?g/kg. The mRNA expression of ANF/ANP in the myocardial tissues of rat embryos of day 15,19 and suckling rats aged 5 days were detected by semiquantitative RTPCR analysis. Results TCDD could upregulate ANF mRNA expression in the myocardial tissues of rat embryos and suckling rats,as compared with control group. Conclusion TCDD can upregulate ANF mRNA expression in myocardial tissues,suggesting that TCDD may cause injuries of cardiac functions of rat embryos and suckling rats.
