RAPID DETERMINATION OF TOTAL VITAMIN E IN LYCOPENE OLEORESIN BY SOLID PHASE EXTRACTION AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
- VernacularTitle:固相萃取-高效液相色谱法快速测定番茄红素油树脂中的总VE
- Author:
Yun ZHENG
;
Guoxiu QI
;
Kexu YAN
;
Jingcheng ZHAO
- Publication Type:Journal Article
- Keywords:
solid phase extraction;
high performance liquid chromatography;
lycopene oleoresin;
total vitamin E
- From:
Acta Nutrimenta Sinica
1956;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a rapid determination method of total VE in lycopene oleoresin.Method:By eliminating saponifiable matters and strong polar materials from the sample,VE was concentrated efficiently by PRP-6 SPE with 90% ethanol/water and 100% ethanol as elution solvents consecutively.The removal of polar materials was satisfactory.VE was determined by HPLC.Results:The linear regression was carried out,the relative coefficient r=0.9998 and 99.85～99.95 % recovery of VE were achieved.Conclusion:A simple and rapid VE determination method with good sensitivity is developed.
