Inhibitory effect of licoric extracts on activity of tyrosinase
- VernacularTitle:甘草提取物对酪氨酸酶活性的抑制作用
- Author:
Jun SHEN
;
Liping HE
;
Ruibin ZHANG
- Publication Type:Journal Article
- Keywords:
tyrosinase;
licorice root;
VitC;
melanin
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2004;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the inhibitory effects of the licoric extracts on the activity of tyrosinase, which is constituted of monophenolase and diphenolase. METHODS: The 50%(V/V) alcohol is used as extractant. To investigate the effects of the licoric extracts on the monophenolase and diphenolase activity, the absorbance of the solution containing several concentrations of the licoric extracts was measured at 475 nm and 390 nm, using the L-tyrosine and pyrocatechin as the substrate, respectively. RESULTS: The licoric extracts has showed a strongly inhibition on the activity of tyrosinase, either monophenolase or diphenolase. The IC_ 50 is 1.94 and 2.94 mg?ml -1 , respectively. CONCLUSION: The licoric extracts has inhibitory effects on both monophenolase and diphenolase. The diphenolase activity test system using pyrocatechin as the substrate is a useful procedure to study the activity of tyrosinase.
