Construction of pancreatic duodenal homeobox-1 recombinant retroviral vector and its stable expression in liver progenitor cells
- VernacularTitle:胰十二指肠同源盒基因-1逆转录病毒表达载体的构建及其在肝干细胞中的稳定表达
- Author:
Caixia JIN
- Publication Type:Journal Article
- Keywords:
pancreatic duodenal homeobox-1;
retroviridae;
liver;
stem cells
- From:
Academic Journal of Second Military Medical University
2000;0(08):-
- CountryChina
- Language:Chinese
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Abstract:
Objective:To construct a recombinant retroviral vector expressing pancreatic duodenal homeobox-1(PDX-1) gene and express the PDX-1 expression cassette in liver progenitor cells(LEPCs),so as to study the conversion of hepatic cells to pancreatic-like cells.Methods: The full length of PDX-1 gene was amplified by PCR and subcloned into pMSCVpuro vector.The pMSCV PDX-1 puro was then introduced into Phoenix package cells and the cell culture supernatant was used for Ping-Pong infection of another packaging cell line PT67 to obtain stable virus-producing cell line.Results: The pMSCV PDX-1 puro vector was successfully constructed and transfected into PT67 cells.The viral supernatants of PT67 cells efficiently infected LEPCs in vitro and the infected LEPCs stably expressed PDX-1 gene.Conclusion: The pMSCV PDX-1 puro vector and PDX-1 expressing LEPCs have been successfully constructed,laying a basis for studying the transdifferentiation of liver progenitor cells to pancreas lineage cells.
