rhCD40L induces mononuclear macrophages secreting matrix metalloproteinases through cyclooxygenase-2 pathway
- VernacularTitle:CD40配体通过诱导型环氧合酶途径诱导单核巨噬细胞分泌基质金属蛋白酶
- Author:
Min FAN
- Publication Type:Journal Article
- Keywords:
CD40 ligand;
cyclooxygenase-2;
matrix metalloproteinases;
macrophages
- From:
Academic Journal of Second Military Medical University
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the influence of rhCD40L on mononuclear macrophage,U937 cells,secreting matrix metalloproteinases(MMPs) and its possible mechanism.Methods: U937 cells were treated with different concentrations of rhCD40L and NS-398(specific antagonist of COX-2) for 24 h and the supernatants were harvested.Cells treated with 0.4 ?g/ml rhCD40L were further treated with 10~(-4) mol/L NS-398 or aspirin separately for 24 h and the supernatants were harvested.Zymography was used to determine the activities of MMPs in the above supernatants.Results: rhCD40L increase the activity of MMP-2 and MMP-9 in a dose-dependent manner(P
