High-performance liquid chromatography/tandem mass spectrometry for determination of cyclosporin A level in whole blood
- VernacularTitle:高效液相色谱-质谱联用测定全血中环孢霉素A含量
- Author:
Bin WANG
- Publication Type:Journal Article
- Keywords:
cycolsporin A;
chromatography,high pressure liquid;
spectrum analysis,mass
- From:
Academic Journal of Second Military Medical University
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a high performance liquid chromatography/tandem mass spectrometry(HPLC-MS) method for determination of cyclosporin A level in the whole blood.Methods: A Hypersil C_(8) column(65 ℃,4.6 mm ?150 mm,5 ?m) was used with a mobile phase of acetonitrile-acetic acid(pH 4.50) 7030 at a flow rate of 1.0 ml?min~(-1).Atomospheric pressure electronic spray ionization was used to quantify cyclosporin A,with the capillary voltage being 4 000 V,the flow of drying gas being 8.0 L?min~(-1),nebulizer pressure being 30 psi,and fragment voltage being 140 V.Results: The calibration curve of cyclosporin A was linear in a range of 26.68-1 067 ?g?L~(-1): y=0.001 1x-0.004 6(r=0.999 3).The lowest limit of detection was 4.35 ?g?L~(-1).The method recoveries of low,medium and high concentrations cyclosporin A were((89.81?4.91)%,)(104.5?3.73)%,and(95.17?2.33)%,respectively;the extraction recoveries of 3 concentrations were(77.64?4.38)%,(78.01?3.79)%,and(83.02?1.59)%,respectively.The intra-day and inter-day RSD was less than 6%,and the RSD was less than 5% in 150 days.Conclusion: The present method is fast,sensitive and selective.It is suitable for clinical monitoring of cycolsporin A.
