Establishment of human orbital rhabdomyosarcoma model in mice
- VernacularTitle:人眼眶横纹肌肉瘤动物模型的建立
- Author:
Li LIANG
- Publication Type:Journal Article
- Keywords:
models,animal;
orbital;
rhabdomyosarcoma
- From:
Academic Journal of Second Military Medical University
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a mouse model of human orbital rhabdomyosarcoma(RD).Methods: RD cells were cultured and implanted subcutaneously into the costal regions of nude mice and severe combined immunodeficiency(SCID) mice(4 weeks old).The nude mice were radiated by ~(60)Co 1 day before the injection.The rhbdomyosarcoma tissue masses were harvested from nude mice and were subcutaneously implanted into the costal regions of another group of BALB/c mice(tumor tissue mass implantation).The tumor growth was observed in all groups and the growth curves were ploted.H-E staining was used to examine pathology of the tumor tissues.Results: Solid tumors were palpable 6-8 weeks after implantation in ~(60)Co radiated nude mice and about 3-4 weeks after implantation in SCID mice.The solid tumors were obviously seen in the nude mice 2 weeks after tumor tissue mass transplantation.The tumor forming rates were 100% in all groups.The tumor growing curves were successfully ploted and the tumors had a progressive growth.Pathological findings of the tumor tissue were similar to those of human orbital rhabdomyosarcoma. Conclusion: The human orbital rhabdomyosarcoma models have been successfully established in nude mice and SCID mice.Tumor tissue mass implantation can shorten the tumor forming time in BALB/c nude mice.This research lays a foundation for further study or human orbital rhabdomyosarcoma.
