Primary hepatocyte culture and its application in drug metabolism and toxicology research and evaluation
- VernacularTitle:原代肝细胞培养及其在药物代谢和毒理学研究中的应用进展
- Author:
Ying DENG
;
Jie CHEN
;
Huichang BI
;
Min HUANG
- Publication Type:Journal Article
- Keywords:
primary culture;
hepatocyte;
drug-drug interaction;
pharmacokinetics;
cytochrome P450
- From:
Chinese Pharmacological Bulletin
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
For more than 40 years primary hepatocytes culture technique has been utilized extensively for assessing effects of drug on metabolizing enzymes (especially cytochromes P450), drug metabolism, drug-drug interactions, and the mechanisms of cytotoxicity and genotoxicity. Human derived primary hepatocytes reserve the metabolism function and enzyme activity of liver. Therefore, this technique has been widely used as a reliable and efficient tool in the drug and xenobiotics evaluation and screen in vitro. This review focuses on primary culture technique of hepatocytes and its application in drug metabolism and toxicology research and evaluation.
