The effect and mechanism of genistein on collagen synthesis of growth plate chondrocytes
- VernacularTitle:染料木黄酮对生长板软骨细胞胶原合成的影响及其机制
- Author:
Yuhua JI
;
Yaoying ZENG
;
Yu YU
- Publication Type:Journal Article
- Keywords:
genistein;
chondrocyte, growth plate;
collagen type Ⅱ;
Sox9
- From:
Chinese Pharmacological Bulletin
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate effects of genistein (5, 7, 4′-trihydroxyisoflavone) on rat costochondral growth plate chondrocyte (RGC) collagen and Sox9 expression. Methods Primary cultured RGC, effects of genistein on collagen synthesis, col2a1 mRNA transcription and Sox9 protein expression of 1st passage RGC were detected by isotope incorporation, RT-PCR and western blotting. Results Genistein inhibited collagen synthesis of 1st passage RGC (P
