An experimental study on the influence of Yinhuang micro-enema compound on the expressions of cytokine and nuclear factor-kappa B
- VernacularTitle:复方银黄微型灌肠剂调节细胞因子表达及核因子-?B信号通路的实验研究
- Author:
Wene CAI
;
Bin YANG
;
Huagang LIU
;
Xiliu ZHANG
- Publication Type:Journal Article
- Keywords:
macrophage;
yinhuang micro-enema compound;
nuclear factor-kappa B;
cytokine
- From:
Chinese Pharmacological Bulletin
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the anti-inflammatory effect of Yinhuang micro-enema compound (YHMEC) in vitro and its possible anti-inflammatory mechanisms. Methods Peritoneal macrophages (PM?) obtained from Wistar rats were randomly divided into four groups: control group, lipopolysaccharide (LPS)-induced group, YHMEC intervention group and dexamethasone intervention group. The morphological changes of cells were observed under convert microscope. Peritoneal macrophage viability was test with MTT. The levels of tumor necrosis factor?(TNF-?) and interleukin-6 (IL-6) were measured using ELISA. The translocation of nuclear factor-kappa B (NF-?B) p65 was detected with immunocytochemical method. Results The levels of TNF-? and IL-6 were increased significantly when PM? were induced by LPS and p65 were translocated from the cell cytoplasm into the nucleus. TNF-?,IL-6 secretions and translocation of NF-?B induced by LPS were inhibited by YHMEC. Conclusion The anti-inflammatory effects of YHMEC may act at least partly through inhititing the translocation of NF-?B and thus depress the expressions of TNF-? and IL-6.
