Oxygen radical absorbance capacity assay and its application
- VernacularTitle:抗氧化能力指数(ORAC)测定原理及应用
- Author:
Jiekun XU
;
Xinsheng YAO
;
Kurihara HIROSHI
- Publication Type:Journal Article
- Keywords:
ORAC;
free radical;
AAPH;
fluorescence;
trolox
- From:
Chinese Pharmacological Bulletin
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To summarize the principles, applications and future development in oxygen radical absorbance capacity (ORAC) assay. Methods The ORAC assay method use Sodium Fluorescein as fluorescent probe, AAPH as free radical initiator, and results are expressed as Trolox equivalents. Results This method has several advantages compared with other methods in linear responses with concentration, specificity, precision, accuracy and ruggedness. Conclusion The ORAC assay has been widely accepted and largely applied to the assessment of free radical scavenging capacity of pure compounds, biological samples, plant and food extracts.
