Effects of GLP-1 treatment on protection of B cells in Otsuka Long-Evans Tokushima fatty rats
- VernacularTitle:胰升糖素样多肽-1对OLETF大鼠胰腺的保护作用
- Author:
Yu WANG
;
Xiaohui GUO
- Publication Type:Journal Article
- Keywords:
Diabetes mellitus;
Insulin;
Glucagon;
Peptides;
Rats,inbred OLETF
- From:
Journal of Peking University(Health Sciences)
2003;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of GLP1 on the blood glucose in type 2 diabetic rats,and its protective effects on the islet B cells.Methods: Thirty rats were divided into three groups: spontaneous type 2 diabetes animal model OLETF rats,GLP-1 [from the twelfth week 56 ?g/(kg?d),sc] therapy group and LETO rats as control.In the 14th and 20th weeks,standard OGTT including fast and 2 h-plasma glucose were measured respectively.In the 14th weeks,3 rats from each group were killed randomly,and the rest of the rats were killed until the 20th week.Immunostaining with the marker of PCNA,TUNEL,and insulin assessed metabolic changes in the islet. Ultrastructure of the B cell was observed with the electronic microscope.Results: In the 14th and 20th week,AUC for insulin were higher in treated animals(10.86?1.56 vs.9.07?1.28,P
