Correlation between microtubule damage and mitochondria damage in hypoxic cardiomyocytes
- VernacularTitle:微管损伤与缺氧心肌细胞线粒体损害的关系研究
- Author:
Yong KUANG
;
Yuesheng HUANG
- Publication Type:Journal Article
- Keywords:
cardiomyocytes;
hypoxia;
microtubule;
confocal laser scanning microscopy;
flow cytometry
- From:Journal of Third Military Medical University
2003;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between microtubule damage and mitochondria damage in hypoxic cardiomyocytes.Methods Cardiomyocytes from neonatal Wistar rat were isolated and cultured for 3-5 d,then divided into three groups: normal control,hypoxia(cultured in hermetic container containing 1%O_(2),5%CO_(2),95%N_(2)),hypoxia with microtubule depolymerization(by adding 4 ?mol/L colchicine).The fluorescence intensity of ?-microtubule was determined by flow cytometry,the morph and distribution of ?-microtubule and mitochondria were checked by confocal laser scanning microscopy(CLSM) at 30 min,1 h after the cadiomyocytes being oxygen deficit.Results As compared with the normal control cells,the fluorescence intensity of ?-microtubule decreased and the microtubule ruptured and distributed confusedly,and the mitochondria changed into disorder in hypoxic cells.As compared with the hypoxic cells,the distribution of mitochondria confused and the fluorescence intensity decreased in the cells of hypoxia with microtubule depolymerization.Conclusion Microtubule damage occurred at the early stage of hypoxia(30 min).Microtubule damage may lead to mitochondria damage in hypoxic cardiomyocytes.
