Effect of taurine on proliferation of cultured pulmonary artery endothelial cells and pulmonary artery smooth muscle cells under hypoxia
- VernacularTitle:牛磺酸对肺动脉内皮和肺动脉平滑肌细胞增殖的双向调节作用
- Author:
Shumin XU
- Publication Type:Journal Article
- Keywords:
taurine;
hypoxia;
pulmonary artery endothelial cells;
pulmonary artery smooth muscle cells
- From:Journal of Third Military Medical University
2003;0(15):-
- CountryChina
- Language:Chinese
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Abstract:
Objective To investigate the effect of taurine and hypoxia(2.5% O_(2) or less than 1% O_(2)) on proliferation of bovine pulmonary artery endothelial cells(PAECs) and pulmonary artery smooth muscle cells(PASMCs).Methods ~(3)H-TdR incorporation method was used to detect the proliferation of PAECs and PASMCs and the proliferation changes by adding taurine.The PAECs and PASMCs were respectively cultured under normoxia(21% O_(2)),hypoxia Ⅰ(2.5% O_(2)),hypoxia Ⅱ(less than 1% O_(2)) and the effect of hypoxia on the cell proliferation was observed in 6,12,24 h.Meanwhile,the two kinds of cells were cultured under normoxia+taurine(0,2.5,5,10,20 mmol/L),hypoxia Ⅰ+taurine,hypoxia Ⅱ+taurine.Results Taurine inhibited the proliferation of PAECs under normoxia in a dose-dependent manner(P20 mmol/L) could inhibit the proliferation of both the cells that grow fast or slow,indicating that taurine may benefit in the prevention and treatment of hypoxic pulmonary hypertension.
