Ultrastructural changes of focal tissue in cerebral cortex of disorders of cortical development in rats
- VernacularTitle:局灶性皮质发育障碍模型鼠大脑皮质病灶组织的超微结构观察
- Author:
Xuntai MA
- Publication Type:Journal Article
- Keywords:
disorders of cortical development,microgyrus;
electron microscopy
- From:Journal of Third Military Medical University
2003;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the pathological characteristics of disorders of cortical development(DCDs) and to analyze the relationship between the characteristics and epileptogenesis.Methods A total of 9 newborn healthy male Wistar rats were randomly divided into three groups including freeze-lesioned,sham-operated and normal groups.The disorders of cortical development model was established by liquid nitrogen lesion.All rats in the three groups were raised for 16 to 18 weeks after operation,then the focal cerebral tissues were taken and observed by transmission electron microscope(TEM) as well as histochemistry(HE and Nissl stain).Results Seizures did not occur in normal rats or sham-operated rats,while in freeze-lesioned rats besides seizures by chance,the main display was more activities,exciting,restlessness,and frequent action of face-wash.EEG of freeze-lesioned rats showed no typical sharp waves,or spikes.All freeze-lesioned rats displayed typical cortical malformation——a longitudinal microgyrus.The electron microscopic examination showed mitochondrial lesions,abnormal neuropil and endoplasmic reticulum expand in neurons and myelinoclasis in neurogliocytes.The changes were more severe around microgyria.Conclusion DCDs lead to main lesion around microgyrus.These changes could be one of mechanisms in an intrinsic epileptogenicity of cortical dysplasias from the pathological characteristics by electron microscopy.
