Effects of janus kinase/singnal transducer and activator of transcription pathway on interferon-gamma in septic rats
- VernacularTitle:感染性休克大鼠JAK/STAT对组织IFN-?表达的影响
- Author:
Fengtong ZHOU
- Publication Type:Journal Article
- Keywords:
sepsis;
interferon-gamma;
signal transduction;
JAK/STAT
- From:Journal of Third Military Medical University
2003;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the effect of janus kinase/signal transducer and activator of transcription(JAK/STAT) pathway on the expressions of interferon-?(IFN-?) in vital organs of rats with sepsis.Methods A sepsis model was induced by cecal ligation puncture(CLP) in 60 male Wistar rats,and randomly divided into CLP group(n=24),AG490 group in which inhibitor of JAK-AG490 at dose of 8 mg/kg was subcutaneously injected 30 min before CLP(n=18),and rapamycin group in which inhibitor of STAT-rapamycin at dose of 0.4 mg/kg was subcutaneously injected 30 min before CLP(n=18).On 2,6,24 h after CLP,all rats were sacrificed,then hepatic and pulmonary tissue samples were harvested to determine IFN-? mRNA expression levels by reverse transcription polymerase chain reaction(RT-PCR) and its protein expression levels by enzyme-linked immunosorbent assay(ELISA).Results As compared with normal rats without CLP,IFN-? mRNA and protein levels in hepatic and pulmonary tissues at 2,6,24 h after CLP significantly elevated(P
