Culture of Blastocystis hominis in vitro in Medium 199
- VernacularTitle:人芽囊原虫在199单相培养基中的体外培养
- Author:
Zhichao WEI
;
Yaqing LI
;
Xu ZHANG
;
Jiying QIAO
- Publication Type:Journal Article
- Keywords:
Blastocystis hominis;
Medium 199;
Vitro culture;
Relevant factors
- From:
Chinese Journal of Schistosomiasis Control
1989;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the culture of Blastocystis hominis in vitro in Medium 199 for further research on the biological characteristics of this intestinal parasite.Methods The growth, reproduction and relevant factors of B.hominis under different culture conditions including pHs, sorts and concentrations of serum, and the number of inoculation were compared. Results The optimal conditions for the continuously anaerobic culture of B.hominis in Medium 199 were as follows: concentrations of new born bovine serum (or human serum and horse serum) ranged from 10%-20%, the number of inoculation was no less than 1?10~5 organisms per tube, pHs ranged from 7.0-8.0, penicillin, streptomycin should be added, anaerobic cultured at 37 ℃.The reproductive peaking-days were 4, 8 at pH 7.0; 3, 6, 9 at pH 7.5 and 2, 4, 7, 9 at pH 8.0. Conclusion B.hominis could be continuously cultured in vitro in Medium 199.
