Inhibitory effect of an small peptide able to interfere with Fc?-receptor recognition on ANCA-accelerated apoptosis of neutrophils
- VernacularTitle:阻断IgG-Fc?R结合短肽在抗中性粒细胞胞浆抗体促进中性粒细胞凋亡中的作用
- Author:
Xiangling WANG
;
Nan CHEN
;
Haijin YU
;
Weiming WANG
;
Liyan NI
- Publication Type:Journal Article
- Keywords:
Antibodies, antineutrophil cytoplasmic;
Vasculitis;
Apoptosis;
Receptors, Fc;
Immunoglobulin G
- From:
Chinese Journal of Nephrology
2005;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigated the inhibitory effect of an IgG-Fc region specific inhibitory peptide on the ANCA-accelerated apoptosis of neutrophils. Methods The peptide was prepared by solid-phase peptide synthesis and its biological activity was identified by rosette formation assay. ANCA was prepared from the sera of active Wegener's granulomatosis (WG) and microscopic polyangiitis (MPA) patients. Neutrophils isolated from the blood of healthy volunteers were primed with TNF-?(2 ng/ml) then incubated with ANCA. At different intervals(3, 6, 12, 18 hours) the neutrophils were harvested to assess the apoptosis by flow cytometric analysis of JC-1 staining, Sub-G1 population and fonnation TUNEL technique. Results Tg19320 bound tightly to human IgG dose-dependendy and inhibited statistically the rosette formation between SRBC-IgG and U937 cells(20.3% vs 53.2% ,P
