Preparation and characterization of polyclonal antibody against N protein of SARS-CoV
- VernacularTitle:抗SARS冠状病毒N蛋白多克隆抗体的制备和鉴定
- Author:
Yang QI
;
Cuirong WEN
;
Shuping CHI
- Publication Type:Journal Article
- Keywords:
SARS virus;
N protein;
antibodies, viral
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare and characterize the polyclonal antibody against N protein of SARS-CoV. Methods The polyclonal antibody against SARS-CoV N was obtained from immunized rabbit with purified GST-N. The titer of the antibody was determined by indirect ELISA, and the specificity by Western blot and immunochemical staining. Results The rabbit′s antibody against SARS-CoV N was prepared successfully. The titer of antiserum against SARS-CoV N was about 1.2?10~ -5 . Western blot and immunochemical staining analysis showed that the polyclonal antibody could bind to the expressed fusion protein specifically. Conclusion The rabbit′s antibody against SARS-CoV N has been prepared successfully, and it can be a useful reagent for clinical diagnosis and further research.
