The injury of myocardium and the roles of oxidative stress on it after limbs ischemia reperfusion in rats
- VernacularTitle:氧化应激在大鼠肢体缺血/再灌注心肌损伤中的作用
- Author:
Lijun ZHAO
;
Shuyun DONG
;
Guoxian DUAN
- Publication Type:Journal Article
- Keywords:
reperfusion injury;
myocardium;
injury
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the injury of myocardium and to investigate the role of oxidative stress after limbs ischemia reperfusion in rats. Methods 20 male healthy Wistar rats were randomly divided into 2 groups: control group(C) and ischemia-reperfusion group (IR). The biochemical methods were used to measure the contents of malondialdehyde (MDA), myeloperoxidase (MPO), ATP, lactic acid (LD) and ATPase of myocardium. The levels of creatine kinase (CK) and creatine kinase MB isoenzyme (CK-MB) in plasma were also observed. The morphologic changes of myocardium were observed with microscope. Results It was found that after rats’ limbs suffering from ischemia-reperfusion, the level of ATP decreased, the activities of three kinds of ATPase (Na~+-K~+-ATPase, Ca~ 2+ ATPase and Mg~ 2+ ATPase) decreased in different degree (P
