Effect of lidocaine on the changes in voltage-dependent sodium channels induced by DNP
- VernacularTitle:利多卡因对DNP引发的电压依赖性钠电流变化的影响
- Author:
Tingting GUAN
;
Xinmin WU
- Publication Type:Journal Article
- Keywords:
lidocaine;
patch-clamp techniques;
hippocampus;
neurons;
voltage-dependent sodium channel
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of lidocaine, the sodium channel blocker, on electrical physiological changes in the circumstances of hypoxia. Methods The effects of different doses of lidocaine (10~ -5 mol/L, 10~ -4 mol/L and 5?10~ -4 mol/L) on the changes in voltage-dependent sodium currents in hippocampal neurons of the rat induced by 10~ -5 mol/L DNP (2,4-dinitrophenol) were recorded by whole-cell configuration of the patch clamp. Results DNP could increase the spike amplitude of sodium currents significantly. With the application of 10~ -5 mol/L and 10~ -4 mol/L lidocaine in advance, the spike values of each group were not significantly different from that of the contral after the administration of DNP, but the spike values after 5?10~ -4 mol/L lidocaine were lower than that of the control after the administration of DNP. Conclusion With the use of DNP, lidocaine can achieve the effect of brain protection through inhibiting the voltage-dependent sodium channel.
