The experimental study on the relationship between proangiogenic factors and the peritubular angiogenesis of diabetic renal
- VernacularTitle:糖尿病鼠肾小管周围新生血管与促血管生长因子关系研究
- Author:
Zejun CHEN
;
Songmin HUANG
;
Yibin YANG
- Publication Type:Journal Article
- Keywords:
Diabetic nephropathy;
VEGF;
Angiopoietin-2;
Angiogenesis
- From:
Chinese Journal of Practical Internal Medicine
2006;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role and significance of vascular endothelial growth factor(VEGF)and angiopoietin-2(Ang-2)in peritubular angiogenesis of diabetic kidney.Methods diabetic rats were induced by Streptozotocin.The expressions of VEGF and Ang-2 in renal tissue of rats were detected by immunohisochemistry.VEGF and Ang-2 mRNA in kidney was also detected by RT-PCR,and quantified by computerimage analysis.Results From 2-week to 24-week,VEGF mRNA level in diabetic renal upregulated continuously compared with control group,with peak level at 16 and 20 weeks.VEGF immunostaining in diabetic renal tubuli increased apparently compared with control group.Ang-2 mRNA in diabetic renal was only detected at 16 and 20 weeks.Ang-2-expressing peritubular microvessel in diabetic renal cortex was found by immunohistochemistry from 12 -week to 24-week with peak level at 16 week.No detecable Ang-2 mRNA and immunostaining were found in control renal.The changes correlated VEGF with Ang-2 in diabetic renal after 12 weeks.Conclusion There are the formation of Ang-2-staining peritubular microvessels in diabetic renal cortex in middle and later stages.VEGF and Ang-2 take part in angiogenesis in diabetic renal.
