Screening differentially expressed genes in human leukemia cell line Jurkat induced by bone marrow stromal cells from leukemia patients
- VernacularTitle:白血病原代骨髓基质细胞诱导Jurkat细胞基因差异表达研究
- Author:
Zhongjun LI
;
Xinghua CHEN
- Publication Type:Journal Article
- Keywords:
bone marrow stromal cells;
gene cloning;
suppression subtractive hybrydization
- From:Journal of Third Military Medical University
2003;0(14):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen and analyze the differentially expressed genes of Jurkat cells cocultured with bone marrow stromal cells(BMSCs) from leukemia patients.Methods Suppression subtractive hybridization(SSH) was employed to establish subtracted cDNA library of differentially expressed genes in Jurkat cells cocultured with BMSCs from leukemia patients in vitro.The cDNA fragments were sequenced and analyzed.Results The differentially expressed gene cDNA library of Jurkat cells cocultured with BMSCs from leukemia patients in vitro was developed by SSH.Primary screening was done by reverse northern hybridization.Thirty up-regulated and 22 down-regulated cDNA fragments were isolated and sequenced,and sequence homology was performed by BLAST in GenBank.These genes were related to cell cycle regulation,cellular apoptosis and energy metabolism.The information of differentially expressed genes in Jurkat cells implied that their secretion function,cell cycle and apoptosis were affected by BMSCs from leukemia patients.Conclusion BMSCs from leukemia patients might influence gene expression of Jurkat cells.The differentially expressed genes might be significantly associated with the protection of BMSCs from leukemia patients on Jurkat cells during Daunorubicin exposure.
