Preliminary Study on Apoptosis of Human Gastric Carcinoma Cell line MKN45 Induced by Arsenic Trioxide and its Molecular Mechanism
- VernacularTitle:三氧化二砷对人胃癌MKN45细胞凋亡的影响
- Author:
Jinjing KE
;
Qinshu SHAO
;
Zhiqiang LING
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
Arsenic trioxide(As2O3);
Apoptosis
- From:
Journal of Medical Research
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the possibility of human gastric carcinoma cells apoptosis induced by arsenic trioxide and its mechanism.Methods After treatment with arsenic trioxide, the cytotoxicity to human gastric carcinoma cells MKN45 was quantified using trypan blue exclusion, and IC 50 was determined. Apoptotic cells were detected with flow cytometry, DNA cytofluorometry, DNA electrophoresis. Results Arsenic trioxide inhibited the growth of human gastric carcinoma cells MKN45 in a dose-dependent manner in a certain range of dose with a IC 50 of (11.05?0.25)?mol/L; Apoptotic peak, characteristic morphologic features of apoptosis and DNA ladder were observed in human gastric carcinoma cells MKN45 treated with 1~10 ?mol/L arsenic trioxide. Conclusions Arsenic trioxide can induce apoptosis of human gastric carcinoma cells MKN45, suggesting a great potential in the treatment of gastric carcinoma.
